Fig 7.
Effects of HDAC1 and -2 depletion on MHV68 replication. (A) Primary macrophages generated and treated as described for Fig. 6 were infected at an MOI of 1 (in the absence of OHT) with wild-type MHV68 and orf36 mutant viruses on day 13 of in vitro culture. RNA was collected at 16 h postinfection, and RTA expression was measured via qRT-PCR and normalized to corresponding GAPDH mRNA levels using the ΔCT method. Data were pooled from 2 to 4 experiments. (B) Primary, OHT-treated macrophages with indicated Cre genotypes were infected at an MOI of 1 with wild-type or N36S MHV68 as described for panel A, and viral yield was measured at the indicated times postinfection. (C) Primary, OHT-treated macrophages with the indicated Cre genotypes were infected at an MOI of 10, and viral DNA accumulation was measured at the indicated time points by real-time PCR. *, P < 0.05. Data are representative of three independent experiments. Error bars represent a standard error of the mean.