Fig 5.

Poly(A)⁺ RNA accumulates in the nucleus in cells infected with WRL-A. HeLa cells were either mock infected or infected with WT KOS, WRL-A, or d2-3 as indicated. Eight hours after infection, cells were fixed, and in situ hybridization was performed by using a biotinylated oligo(dT) probe to detect poly(A)⁺ RNA. ICP27 was detected by staining with anti-ICP27 antibody. In the bottom row, mock-infected cells were treated with RNase.