Fig 6.
CK2 phosphorylation inactivates DNA binding in HPV-11 and HPV-31 E1. (A) DNA pulldown assays were performed using GST HPV-11 E12–353, a DNA probe representing the BPV ori and a nonspecific probe containing sequences from E1. The assays were performed in the absence or presence of 50 U of CK2 as indicated. (B) Pulldown assays of the BPV ori fragment were performed with the indicated N-terminal fragments of E1 from HPV-11 and HPV-31, in the absence and presence of 50 U of CK2. (C) A cartoon illustrating the GST fusion constructs used in these assays.