Skip to main content
. Author manuscript; available in PMC: 2014 Jun 10.
Published in final edited form as: Cancer Cell. 2013 Jun 10;23(6):826–838. doi: 10.1016/j.ccr.2013.05.002

Figure 3. SYK and PI3K-dependent signaling in DLBCLs with low baseline NF-κB.

Figure 3

(A, B) DHL4, DHL6 and LY7 cell lines were retrovirally transduced with mAKT or pMIG vector, FACS-sorted for GFP expression, treated for 24 hr with R406 or vehicle, then analyzed for p-AKT(S473) and total AKT by immunoblotting (A) and for proliferation (B). (C) DLBCL cell lines were incubated with R406 or vehicle for 1 hr, BCR cross-linked (BCR) or left untreated, fixed, then stained with DAPI (blue) and with Cy3-conjugated anti-FOXO1 antibody (red) (left panels). Scale bar represents 10 μm. The mean nuclear staining intensity of Cy3-conjugated-FOXO1 was quantified for each cell and plotted against its mean DAPI staining intensity (right panels). (D) The transcript abundance of FOXO1 target genes, p27 and BIM, in DLBCL cell lines treated with R406 or vehicle for 24 hr was assessed by qRT-PCR relative to PPIA. p-values for mAKT vs. pMIG (control) at each timepoint (B) and for vehicle vs. R406 (D) were determined with a one-sided Welch t-test. ***, p≤0.0001; **, p≤0.001. Error bars represent the SD of 3 independent assays in a representative experiment. See also Figure S3.