Fig. 7.

Co-localization of VWF and P-selectin in a mature MK from an untreated WT mouse. Representative sections from a WT mouse spleen were double stained for P-selectin (P, large gold particles) and VWF (W, small gold particles). A representative stage III MK is shown in a) and co-localization of P-selectin and VWF in three areas of its cytoplasm are shown in b–d). Red arrows and arrowheads point to structures containing the proteins, while the white ones point to the structure itself. As seen in (a), the mature MK is characterized by a multilobed nucleus and abundant cytoplasm with many α-granules in the perinuclear zone. As seen in b–d), VWF and P-selectin appear separately in the mature WT MK. Both proteins were found either dispersed in the cytoplasm or adjacent to the DMS (whole arrows). P-selectin was found also in the α-granule membrane (arrowheads), while VWF was localized to one pole of the granule. Original magnifications: a) × 4,400; b,c,d) × 30,000.