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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2013 Mar 29;269(2):89–99. doi: 10.1016/j.taap.2013.03.015

Fig.1.

Fig.1

Effect of quercitrin on apoptosis and apoptotic proteins induced by UVB exposure. A, B, and E, JB6 cells were pretreated with quercitrin (10, 20, and 40 μM) or acetone for 1 h prior UVB exposure. After 24 h, the cells were collected for apoptosis analysis using flow cytometry (A and B) or for immunoblotting assay (E). C and D, 6–8 week old female SKH-1 mice dorsal skin was topically administrated with either aceton (control group) or quercitrin one day before UV exposure. The mice were then exposed to 75 mJ/cm2 of UVB for 3 times per week up to 6 weeks. At 1 day and 7 days, the animals were euthanized and dorsal skin tissues were isolated and subjected for immunofluoresence staining of apoptosis (C and D). The positive cells were counted from a total of 500 cells from 8 random field using Olympus BX51 microscope. The results were expressed as percentage of TUNEL-positive cells (apoptosis index). F, The same as C and D, but mouse dorsal skin tissues were isolated and total protein was extracted for examination of expression levels of cleaved PARP-1 and cleaved caspase-3. * indicates a significant difference compared with control without UVB exposure (p<0.05). # indicates a significant difference compared with 240 mJ/cm2 UVB exposure (p<0.05).