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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2013 Mar 29;269(2):89–99. doi: 10.1016/j.taap.2013.03.015

Fig. 6. Quercitrin inhibits ROS generation induced by UVB exposure.

Fig. 6

A, B, C, and D, JB6 cells were pretreated with quercitrin (10, 20, and 40 μM) or acetone for 1 h prior UVB exposure. After 2 h, the cells were subjected for measurement of O2•− (A and B) and H2O2 (C and D). E, 2×106 JB6 cells were collected for measurement of •OH generation. (a) Electron spin resonance (ESR) spectrum recorded 2 minutes from the mixture of JB6 cells and 100 mM 5,5-dimethyl-1-pyrroline 1-oxide (DMPO); (b) the same as (a) but the cells were exposed to 240 mJ/cm2 UVB exposure; (c), The same as (a) the cells were pretreated with 10 μM quercitrin for 1 h before ESR measurement; (d), (e), and (f) the same as (b) but the cells were pretreated with different concentration of quercitrin (10, 20, and 40 μM) 1 h prior to UV exposure. * indicates a significant difference compared with control without UVB exposure (p<0.05). # indicates a significant difference compared with 240 mJ/cm2 UVB exposure (p<0.05).