DCs from MRL/lpr mice are defective in IL-6 secretion upon TLR4, 7, and 9 stimulation, but not upon TLR3 stimulation. 1 × 104 BMDCs (A) or 1 × 105 ex vivo splenic DCs (B) were stimulated with LPS (30 μg/ml). 5 × 105 BMDCs were stimulated with poly(I:C) (50 μg/ml) (C), R848 (10 μg/ml) (D), and non-CpG ODN (●/□) or CpG ODN (●/○) (1 μg/ml) (E). IL-6 was quantitated by ELISA from the day 4 culture supernatants. Data represent at least 5 mice per group. (●, B6; ○, MRL/lpr; ▲, MRL; △, B6.lpr; ◆, NZM2410; ◇, NZBxNZW F1).