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. Author manuscript; available in PMC: 2013 Jul 3.
Published in final edited form as: J Immunol. 2007 Apr 15;178(8):4803–4810. doi: 10.4049/jimmunol.178.8.4803

FIGURE 6.

FIGURE 6

DCs from MRL/lpr mice fail to activate NF-κB and AP-1. BMDCs were stimulated with LPS (15 μg/ml) for the indicated times. Nuclear extracts were prepared, and NF-κB/DNA binding (A) or AP-1/DNA binding (D) was assessed by EMSA. Nuclear extracts prepared from unstimulated B6 BMDCs (lane 1) or from DCs stimulated 6 h with LPS (lanes 2–4) were incubated with radiolabeled DNA probe (lanes 1–4), unlabeled competitive DNA (lane 3), or mutant DNA (lane 4), and NF-κB DNA binding was assessed by EMSA (B). NF-κB/DNA complexes in the nuclear extracts from unstimulated B6 DCs (lane 1) or from DCs stimulated 6 h with LPS (lane 2–5) were supershifted using p65 (lane 3), p50 (lane 4), or c-Rel antiserum (lane 5) (C).