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. Author manuscript; available in PMC: 2013 Jul 3.
Published in final edited form as: J Immunol. 2007 Apr 15;178(8):4803–4810. doi: 10.4049/jimmunol.178.8.4803

FIGURE 8.

FIGURE 8

In addition to IL-6, other soluble factors regulate autoantibody secretion. Purified B cells (1 × 105) from 2-12H/Vκ8 mice were stimulated with LPS (30 μg/ml) in the absence (▲) or presence of DC CM (25% of final volume) from B6 (●) or MRL/lpr (○) mice (A). DC CM from individual MRL/lpr mice (○) was untreated or neutralized with anti-IL-6 Ab (50 μg/ml) before coculture with B cells from 2-12H/Vκ8 mice (B). 5 × 104 purified B cells from 2-12H/Vκ8 and B6 mice were stimulated with LPS (30 μg/ml) in the absence (▲) or presence of DC CM from B6 (●) or MRL/lpr (○) mice (C). Secreted IgMa/κ levels were quantitated by ELISA from the day 4 culture supernatant. LPS-stimulated purified B cells (100%) secreted 1–10 μg/ml IgMa/κ. Data represent 15 (A), 5 (B), and 4 (C) MRL/lpr mice.