TABLE 1.
Genotyping
| Alleles | Primers | Conditions | Products |
|---|---|---|---|
| Tcfap2aki7lacZ versus | Alpha 6/7 5′-GAA AGG TGT AGG CAG | 45 s at 95°C, 1 min at 67°C, | Tcfap2aki7lacZ 300 bp |
| Tcfap2a+ | AAG TTT GTC AGG GC -3′ | 1 min 10 sec at 72°C | |
| Alpha 3′KO 5′-CGT GTG GCT GTT | for 33 cycles | ||
| GGG GTT GTT GCT GAG GTA C -3′ | |||
| IRESUP 5′-GCT AGA CTA GTC TAG | Tcfap2a+ 500 bp | ||
| CTA GAG CGG CCC GGG -3′ | |||
| Tcfap2b− versus | 4 Exon DW 5′-CCT CCC AAA TCT | 45 s at 95°C, 45 s at 58°C, | Tcfap2b−380 bp |
| Tcfap2b+ | GTG ACT TCT-3′ | 1 min at 72°C for 37 cycles | |
| PGK-PolyA DW 5′-CTG CTC TTT ACT | |||
| GAA GGC TCT TT-3’ | |||
| 4 Exon Rev 5′-TTC TGA GGA CGC | Tcfap2b+ 221 bp | ||
| CGC CCA GG-3′ | |||
| α-Cre transgene | Cre1 5′-GCT GGT TAG CAC CGC | 45 s at 95°C, 1 min at 67°C, | Presence of α-Cre |
| AGG TGT AGA G-3′ | 1 min 10 sec at 72°C | transgene 420 bp | |
| Cre3 5′-CGC CAT CTT CCA GCA | for 33 cycles | ||
| GGC GCA CC-3′ | |||
| Tcfap2alox versus | Alflox4 5′-CCC AAA GTG CCT | 45 s at 95°C, 45 s at 65°C, | Tcfap2alox 560 bp |
| Tcfap2a+ | GGG CTG AAT TGA C-3′ | 1 min at 72°C for 39 cycl | |
| Alfscsq 5′-GAA TCT AGC TTG | Tcfap2a+490 bp | ||
| GAG GCT TAT GTC-3′ |