Cyp7a1-tg mice showed increased hepatic VLDL secretion but maintained plasma TG homeostasis. Female mice were fed an HFD for 4 months. (A) Mice were briefly fasted for 4 hours, and the hepatic VLDL secretion was assayed after an intraperitoneal injection of taloxapol. (B,C) Hepatic mRNA and protein expression of MTP in mice fed for 4 hours after overnight fasting. Liver samples were pooled from six mice per group for western blot analysis of MTP protein. (DF) Plasma cholesterol, TG, and FFA levels in mice fasted overnight. Results are expressed as means and standard errors (n =6–8). *Statistically significant difference (P < 0.05) versus chow-fed WT mice; **statistically significant difference (P < 0.05) versus HFD-fed WT mice. Abbreviations: Tg, transgenic; WT, wild-type.