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. Author manuscript; available in PMC: 2013 Jul 3.
Published in final edited form as: Hepatology. 2010 Aug;52(2):678–690. doi: 10.1002/hep.23721

Fig. 6.

Fig. 6

Cyp7a1-tg mice showed increased hepatic glycogen storage. (A) Quantification of the hepatic glycogen content in overnight fasted female mice. (B) Female mice were fed chow or an HFD for 4 months. Hepatic glycogen was determined by PAS staining of liver sections from female mice fasted overnight. (C) Female mice on an HFD were fasted for 16 hours and refed for 6 hours. AKT was immunoprecipitated from pooled liver samples (n =6); total and phosphorylated AKT was detected by WB. (D) Hepatic GSK3β and phosphorylated GSK3β were detected by WB in pooled liver samples (n =6) from refed mice. Results are expressed as means and standard errors (n =6–8). *Statistical significance (P < 0.05) versus WT mice on a chow diet; **statistically significant difference (P < 0.05) versus HFD-fed WT mice. Abbreviations: IP, immunoprecipitation; Tg, transgenic; WB, western blotting; WT, wild-type.