Table 1.
Hepatic mRNA Expression in WT and CYP7A1-tg Mice
| WT + Chow | Tg + Chow | WT + HFD | Tg + HFD | |
|---|---|---|---|---|
| Fatty acid metabolism | ||||
| SREBP-1 | 1 ± 0.44 | 2.07 ± 0.16* | 10.46 ± 0.77† | 3.49 ± 0.62* |
| SCD-1 | 1 ± 0.22 | 1.86 ± 0.5 | 18.45 ± 1.8† | 2.6 ± 0.63* |
| FAS | 1 ± 0.33 | 3 ± 0.3* | 0.8 ± 0.05 | 2.4 ± 0.5* |
| ACC | 1 ± 0.2 | 1.65 ± 0.23 | 1.24 ± 0.14 | 1.49 ± 0.22 |
| CD36 | 1 ± 0.07 | 0.77 ± 0.08 | 1.65 ± 0.13† | 0.86 ± 0.15* |
| Cholesterol metabolism | ||||
| HMG-CoA reductase | 1 ± 0.53 | 11.18 ± 2.29* | 0.73 ± 0.06 | 19.6 ± 6.82* |
| LDLR | 1 ± 0.13 | 3.04 ± 0.4* | 1.27 ± 0.1 | 3 ± 0.55* |
| SREBP-2 | 1 ± 0.2 | 2.48 ± 0.39* | 0.97 ± 0.07 | 2.79 ± 0.29* |
| Bile acid metabolism | ||||
| mCYP7A1 | 1 ± 0.27 | 0.006 ± 0.003* | 2.28 ± 0.21† | 0.05 ± 0.03* |
| mCYP8B1 | 1 ± 0.22 | 0.03 ± 0.01* | 0.29 ± 0.04† | 0.02 ± 0.008* |
| SHP | 1 ± 0.4 | 3.08 ± 0.9* | 0.92 ± 0.12 | 1.99 ± 0.4* |
| rCyp7a1 | ND | Ct = 19.4 ± 0.27 | ND | Ct = 19.8 ± 0.29 |
| Inflammatory cytokines | ||||
| TNFα | 1 ± 0.1 | 1.37 ± 0.38 | 3.35 ± 0.39† | 1.6 ± 0.2* |
| IL6 | 1 ± 0.09 | 0.94 ± 0.18 | 1.54 ± 0.24 | 0.73 ± 0.09* |
| TGFβ1 | 1 ± 0.09 | 1.3 ± 0.3 | 2.47 ± 0.18† | 1.05 ± 0.07* |
Female mice were on chow or HFD for 4 months. The results are shown as means and standard errors (n = 6–10).
Abbreviations: ACC, acetyl coenzyme A carboxylase; CYP8B1, cholesterol 12α-hydroxylase; HMG-CoA, 3-hydroxy-3-methyl-glutaryl-coenzyme A; IL6, interleukin-6; LDLR, low-density lipoprotein receptor; ND, not determined; SCD-1, stearoyl-coenzyme A desaturase 1; Tg, transgenic; TGFβ1, transforming growth factor β1; TNFα, tumor necrosis factor α; WT, wild type.
*
Statistically significant difference (P < 0.05) versus WT mice on the same diet.
†
Statistically significant difference (P < 0.05) versus WT mice on chow.