Table 1.
Primers and Probes Used for PCR, RT-PCR, and Real-Time PCR of Tick-Borne Pathogen Nucleic Acid Detection
| Primer or probe name | Sequences of oligonucleotide probes and primers (5′→3′) | References |
|---|---|---|
| TBEV | ||
| 283F1 | GAGAYCAGAGTGAYCGAGGCTGG | Skarpaas et al. 2006 |
| 827R1 | AGGTGGTACTTGGTTCCMTCAAGT | |
| 349F2 | GTCAAGGCGKCTTGTGAGGCAA | |
| 814R2 | TTCCCTCAATGTGTGCCACAGG | |
| F-TBE1 | GGGCGGTTCTTGTTCTCC | Schwaiger and Cassinotti 2003 |
| R-TBE1 | ACACATCACCTCCTTGTCAGACT | |
| TBE-Probe-WT | TGAGCCACCATCACCCAGACACA | |
| B. burgdorferi s.l. | ||
| NC1 | CCTGTTATCATTCCGAACACAG | Rar et al. 2005 |
| NC2 | TACTCCATTCGGTAATCTTGGG | |
| NC3 | CTGCGAGTTCGCGGGAGA | Postic et al. 1994 |
| NC4 | TCCTAGGCATTCACCATA | |
| A. phagocytophilum | ||
| ApMSP2f | ATGGAAGGTAGTGTTGGTTATGGTATT | Courtney et al. 2004 |
| ApMSP2r | TTGGTCTTGAAGCGCTCGTA | |
| ApMSP2 (probe) | FAM-TGGTGCCAGGGTTGAGCTTGAGATTG-TAMRA | |
TBEV, tick-borne encephalitis virus.