Th2 differentiated CD4 T cells are reduced in the lymph nodes of EC-OVA sensitized MIF-KO mice. (A) OVA-stimulation increases the number of IL-4 producing CD4 T-cells, measured by flow cytometry, to a greater degree in the WT, compared to MIF-KO system. (B&C) Both Th1 and Th2 differentiation, measured by upregulation of transcription factors Tbet and Gata3, are decreased in MIF-KO lymph nodes. Unsensitized (n=3), WT (n=3), MIF-KO (n=3). Summary data are pooled from three independent expeirments *** p<0.001, **p<0.01, *p<0.05.