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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Dig Dis Sci. 2013 Apr 5;58(7):1899–1908. doi: 10.1007/s10620-013-2648-3

Figure 1. Iron overload decreases hepatocyte insulin response. A. Representative western blot.

Figure 1

Control (8-hydroxyquinoline only) and iron loaded (50 μM iron with 8-hydroxyquinoline) AML-12 cells were stimulated with 10 nM insulin and analyzed by western blot for phospho-PKB. GAPDH was analyzed as loading control. B. Relative insulin response. The insulin-stimulated phospho-PKB signals from n=18 experiments were quantified relative to GAPDH as described under Methods and normalized to control cells in each experiment; the means +/− se are plotted (*** p<0.001).