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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Dig Dis Sci. 2013 Apr 5;58(7):1899–1908. doi: 10.1007/s10620-013-2648-3

Figure 2. Triglycerides accumulate in hepatocytes treated with free fatty acids.

Figure 2

Cells loaded with vehicle only (control), 200 μM stearic acid, or 200 μM oleic acid were stained with Oil Red O as described under Methods. A. Representative micrographs. Each field contains approximately 300 cells treated for 24 hours (400x). B. Time course of lipid accumulation. After staining, the % cell area occupied by lipid vacuoles was quantified by photodensitometric analyses of six fields per condition. Data represent the means +/− se of n=3 experiments.