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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Dig Dis Sci. 2013 Apr 5;58(7):1899–1908. doi: 10.1007/s10620-013-2648-3

Figure 8. Curcumin prevents iron-induced insulin resistance and oxidative stress in cells treated with stearic acid.

Figure 8

Cells were treated for 3 days (+/− 200 μM stearic acid, 18:0) followed by 2 hours (+/− 200 μM stearic acid and 50 μM iron, +/− 50 μM 50 μM C3 complex® curcumin + 8-hydroxyquinoline) and tested +/− 10 nM insulin. A. Representative western blots. Samples from a single experiment were analyzed for phospho-PKB, phospho-jnk, phospho-p38, and GAPDH. B. Insulin response. The relative insulin response was determined by phospho-PKB western blot. The means +/− se from n=3 experiments are shown after normalizing to control cells. C. Relative phospho-jnk. The means +/− se from n=3 experiments (as for panel B) are shown for phospho-jnk after normalizing to the iron only samples. D. Relative phospho-p38. Results for phospho-p38 are shown as for panel C. (*** p<0.001 vs. control; ** p<0.01; n.s. not significant).