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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Thromb Haemost. 2013 May 16;110(1):53–61. doi: 10.1160/TH13-03-0184

Figure 4.

Figure 4

Comparisons of the activation rates of recombinant FX-WT and FX-Thr211Pro by FVIIa. (A) In the absence of TF, FVIIa (100 nM) was added to increasing concentrations of FX-WT (○) and FX-Thr211Pro (●) on PC/PS vesicles (50 μM) in TBS/Ca2+ at room temperature. After 20 min incubation, the reactions were terminated by EDTA and the rate of FXa generation was measured from the cleavage rate of SpFXa as described under “Materials and methods”. (B) The same as (A) except that FVIIa (0.05 nM) in complex with relipidated dcTF (2 nM) was incubated with FX derivatives for 2 min. Data are derived from three independent measurements (±SD).