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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Thromb Haemost. 2013 May 16;110(1):53–61. doi: 10.1160/TH13-03-0184

Figure 5.

Figure 5

Comparisons of the activation rates of recombinant FX-WT and FX-Thr211Pro by FIXa. (A) In the absence of FVIIIa, FIXa (100 nM) was incubated with increasing concentrations of FX-WT (○) and FX-Thr211Pro (●) on PC/PS vesicles (50 μM) in TBS/Ca2+. After 20 min incubation at room temperature, the reactions were terminated by EDTA, and the rate of FXa generation was measured as described under “Materials and methods”. (B) The same as (A) except that FIXa (0.1 nM) in complex with FVIIIa ( 25 nM) was used for the activation of the zymogens for 2–5 min. Data are derived from three independent measurements (±SD).