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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Leuk Res. 2013 May 10;37(8):974–979. doi: 10.1016/j.leukres.2013.04.018

Figure 3.

Figure 3

Inhibition of XIAP decreases proteasome activity. A. 20S proteasome activity is decreased in HL-60 cells treated for 24 hours with ASO or bortezomib, and further decreased by their combination. Data are the result of 3 experiments. Bars indicate the standard error of the mean. Asterisks indicate significant (p < 0.05) differences from treatment with the NSO control plus DMSO. B. An apoptosis assay shows no differences between treatments at 24 hours and apoptosis induction with ASO at 48 hours. The comparisons were between treated groups with NSO control in cells positive for either Annexin V (AnnV) or 7-AAD. The degree of apoptosis seen in the control is likely attributable to the effects of electroporation. C. Western blotting shows increases in IκBα, HSP70, and Mcl-1 levels in HL-60 cells treated for 24 hours with ASO or bortezomib, and a further increase by their combination. Downregulation of XIAP by ASO is confirmed. hr, hour.