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. 2013 May 14;162(3):1539–1551. doi: 10.1104/pp.113.216556

Figure 1.

Figure 1.

Analysis of the expression of PHOT1 and PHOT2 genes in the hypocotyl of Arabidopsis etiolated seedlings by RT-PCR, qRT-PCR, and western blotting. A, RT-PCR analysis of PHOT1 and PHOT2 gene expression levels in wild-type (WT), phot1, phot2, and phot1phot2, PHOT1-OX (transgenic PHOT1 in the wild type), PHOT2-OX (transgenic PHOT2 in the wild type), phot1 PHOT2-OX (transgenic PHOT2 in phot1), and phot2 PHOT1-OX (transgenic PHOT1 in phot2) plants. B, qRT-PCR analysis of PHOT1 and PHOT2 gene expression levels in wild-type, mutant, and transgenic plants. C, Western-blot analysis of phot1, phot2, and the plasma membrane H+-ATPase in the hypocotyl of Arabidopsis etiolated seedlings. To determine phot1 and phot2, 40 μg (for phot1) or 60 μg (for phot2) of protein was loaded in each lane. For the plasma membrane H+-ATPase, 40 μg of protein was loaded in each lane. The numbers on the left indicate molecular masses. Experiments conducted three times on different occasions gave similar results. [See online article for color version of this figure.]