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. Author manuscript; available in PMC: 2014 Jun 27.
Published in final edited form as: Immunity. 2013 Jun 6;38(6):1116–1128. doi: 10.1016/j.immuni.2013.02.022

Figure 3. Normal Tregs display the Foxp3 pro-apoptotic protein signature.

Figure 3

(A and B) Protein immunoblots of conventional (Foxp3−CD25−) and regulatory (Foxp3+CD25+) CD4SP thymocytes from B6 (A) and hBcl-2Tg mice (B) containing the Foxp3-GFP reporter (Bettelli et al., 2006). Puma mRNA (A lower panel) was determined by quantitative RT-PCR relative to 18S rRNA (mean ± SE of triplicate samples). Data in A are representative of 2 experiments and data in B of 4 experiments.

(C) Intracellular mBcl-2 expression in Treg subpopulations (Xwt and Xsfy) in the thymus of heterozygous B6xScurfy female mice expressing the Foxp3GFP reporter. Dashed lines represent negative control staining. Data are representative of 2 experiments.

(D) Frequency (left) and relative number of Foxp3+CD4SP thymocytes compared to B6 (right). Mean ± SE of 5-10 mice per group.

*, p<0.01; **, p<0.001; ***, p<0.0001.

See also Figure S3.