Figure 2.

Distribution of AAV-orexin injection sites in Atx mice. Each mouse received a unilateral injection into the right side of the hypothala-mus, but injection sites are depicted on either side for clarity. The drawn injection site boundaries encompass approximately 90% of the orexin-immunoreactive neurons. AAV, adeno-associated viral vector; Atx, transgenic (ataxin); DMH, dorsomedial nucleus; Arc, arcuate nucleus; PMV, ventral premammillary nucleus; SuMM, medial supramammillary nucleus; LH, lateral hypothalamus; MM, medial mammillary nucleus; mt, mammillothalamic tract; 3V, third ventricle; TMN, tuberomammillary nucleus. Injected sites are plotted on drawings adapted from Paxinos and Franklin.⁵⁰