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. Author manuscript; available in PMC: 2013 Aug 3.
Published in final edited form as: Cell. 2012 Aug 3;150(3):521–532. doi: 10.1016/j.cell.2012.05.048

Figure 3. bmb encodes a conserved novel predicted transmembrane protein.

Figure 3

(A) Recombinants over total meiotic events are indicated parenthetically in red below the marker name. Candidate genes eliminated based on their WT cDNA sequence are indicated with a red X. A histone gene cluster is indicated as red boxes. Two predicted transposable elements are indicated by black boxes. (B) ORF1.8 encodes a novel 612 residue protein with a predicted N-terminal signal peptide sequence (SP) followed by a Bmb Homology Domain (BHD), a coiled-coil (CC) and two C-terminal transmembrane domains (TM) initiating at amino acid residues 363 and 415 (TMpred program) (Hofmann and Stoffel, 1993). Yeast Kar5p is 27% similar (excluding the C-terminal region) to the D. rerio homolog. The red bar corresponds to the recombinant protein used to generate Bmb antibodies. (C) Rescued bmb− embryos at 50% epiboly (5.5 hpf) and 24 hpf. See also Figure S1.