(A) Bmb protein localizes as distinct foci to the mitotic spindle region during
metaphase (n=4). (B) During anaphase Bmb is interspersed between the separating
chromosomes (inset; n=3) and in the region of the mitotic spindle. (C) Later in
anaphase, Bmb foci are decorating the chromosomes (inset; n=3). (D) At the 2
min time point Bmb surrounds the individual chromosomes, which are still separating (n=5).
(E) Schematic summarizing Bmb localization during metaphase to anaphase/telophase.
Arrows in the right panel indicate that the karyomeres will continue to move to their final central
position in the cell. 0 min corresponds to metaphase at the 32-to-64 cell transition. Bmb (green),
microtubules (DM1a, red), chromosomes (DAPI, white and blue in merge). Scale bar = 5
μm and insets show 2X enlargements. See also Figure S2.