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. 2013 Jul 3;2:8. doi: 10.1186/2046-2530-2-8

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Copyright © 2013 O'Connor et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Sstr3::GFP labeled cilia in the kidney. Sstr3::GFP signal (green) in (A) fixed cryosections of the kidney, (B) isolated EIIa Cre; Cilia^GFPtubules, (C) in situ in the proximal tubule of an anesthetized mouse. Immunofluorescence staining of (D) isolated tubules and (E) kidney sections and Sstr3:GFP localization. Acetylated α-tubulin (red) labeling was used to validate cilia localization of the Sstr3::GFP fusion protein. Nuclei are labeled with Hoescht (blue). Arrows indicate examples of cilia that are double-labeled.