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. Author manuscript; available in PMC: 2014 May 15.
Published in final edited form as: Dev Biol. 2013 Mar 17;377(2):319–332. doi: 10.1016/j.ydbio.2013.03.012

Figure 4. The WAVE/SCAR component GEX-3 localizes to similar regions as endocytosis proteins and is required for the distribution of GFP::hTfR.

Figure 4

A. Subcellular distribution of GEX-3::GFP at apical and basal regions of the intestine. GEX-3::GFP localization relative to apical terminal web (a-IFB-2), an apical membrane-bound protein (a-ERM-1), and the basal plasma membrane protein LET-413/Scribble. The solid white squares mark the regions enlarged on the right.

B. Subcellular distribution of GEX-3::GFP relative to regulators of CME. Relative distribution of GFP::CHC-1, APA-2, GFP::RAB-5, GEX-3::GFP and PH::GFP. The arrows indicate the basal membrane at the junction between two intestinal cells.

C. Effect of GEX-3 on GFP::hTfR localization. The localization of GFP::hTfR is compared to that of APA-2 and RAB-5 in wild type animals and in animals depleted of GEX-3 via RNAi. White arrows indicate regions of overlap. The graphs document the number of puncta expressing both markers within a given rectangular region.