Figure 3.

A. MC65 cells were seeded in 96-well plate in mediumwith (Tet+) or without (Tet-) tetracycline in the presence of the inhibitors at Day 0. The cells were cultured for an additional 72 h and then cell viability was assessed with an MTT assay with data presented as % (SEM) of Tet+ cultures with vehicle. Inhibitors were SC-51089 (SC, 20 μM), nimodipine (Nim, 25 μM), or 2-aminoethoxy-diphenyl borate (2APB, 20 μM). Two-way ANOVA had P<0.0001 for Tet+ vs. Tet-, for agents, and for interaction between these two terms. Bonferroni-corrected post-tests Tet- with Vehicle, SC, or 2APB. B. Murine primary cerebral cortical neurons were incubated with fresh medium (Med, clear bar) or medium that contained 20 μMAβ1–42 (filled bars) and the inhibitors for 48 h. Cell viability was assessed with an MTT assay. SC-51089 protected neurons from Aβ1–42 toxicity at all concentrations used (P<0.001 compared to vehicle for all concentrations of SC-51089) with the greatest protection provided at 50 μM; however, protection by SC-51089 was partial and significantly less than nimodipine (P<0.01 for SC-51089 at 10 or 25 μM, and P<0.05 for SC-51089 at 50 μM). One-way ANOVA had P<0.0001 with Bonferroni-corrected multiple paired comparisons having *P<0.001 compared to Med and ⁺P<0.001 compared to Veh