Table 2.
Effect of A-FOS on GR binding to the GR motif and 1-bp variants
|
GR Motifs |
#in Genome |
#in Peaks(%of Peaks with the Motif) |
%With AP-1(%in c-Jun Peaks) |
+AP-1 |
---AP-1 |
|---|---|---|---|---|---|
| %A-Fos | %A-Fos | ||||
| |
|
Pre-Programmed (5,865 Peaks) |
|||
|
G-ACA---TGT-C |
27,176 |
523 (9%) |
23% (80%) |
44% |
15% |
|
G-ACA---CGT-C |
7,394 |
131 (2%) |
23% (80%) |
38% |
20% |
|
1-bp Variants |
575,842 |
1,719 (30%) |
31% (86%) |
54% |
28% |
| |
|
Re-Programmed (1,946 Peaks) |
|||
|
G-ACA---TGT-C |
27,176 |
665 (34%) |
14% (44%) |
45% |
9% |
|
G-ACA---CGT-C |
7,394 |
134 (7%) |
10% (23%) |
39% |
4% |
|
1-bp Variants |
575,842 |
806 (41%) |
18% (33%) |
50% |
11% |
| |
|
Un-Programmed (425 Peaks) |
|||
|
G-ACA---TGT-C |
27,176 |
205 (48%) |
6% (0%) |
58% |
8% |
|
G-ACA---CGT-C |
7,394 |
29 (7%) |
7% (0%) |
50% |
4% |
| 1-bp Variants | 575,842 | 186 (44%) | 9% (6%) | 41% | 11% |
Effect of A-FOS on GR binding to the GR motif and 1-bp variants depends on the presence of an AP-1 motif within 150-bps. GR ChIP-seq peaks are placed into three groups, pre-programmed, re-programmed, and un-programmed. Column 1: GR motifs. Column 2: # of occurrences of each motif in the genome. Column 3: # of motifs bound by GR. In brackets is the % of peaks that contain the motif. Column 4: % of peaks containing a GR motif containing an AP-1 motif within 150-bps. In brackets is the % of GR motifs contain an AP-1 motif that are bound by c-Jun. Column 5: % of GR motifs containing an AP-1 motif nearby that loses GR binding after inhibition of c-Jun binding by the dominant negative A-FOS. Column 6: % of GR motifs without a nearby AP-1 motif that lose GR binding after inhibition of c-Jun binding by the dominant negative A-FOS.