. 2013 Jun 7;10:15. doi: 10.1186/1742-6405-10-15

Table 2.

Susceptibility and phenotypic properties of V2, V3 and V4 single, double and triple mutants against maraviroc

Loop	Mutant	Tropism	IC₅₀	IC₅₀	MPI
Loop	Mutant	Tropism	IC₅₀	FC increase	MPI
Parental clone	Wild type	R5	1.47 nM	-	-
V2	V169M	R5	1.82 nM	1.23	96%
V3	L317W	R5	2.24 nM	1.52	95%
V4	I408T	R5	5.46 nM	4.00	85%
V2-V3	V169M	R5	1.92 nM	1.30	94.3%
V2-V3	L317W	R5	1.92 nM	1.30	94.3%
V2-V4	V169M	R5	5.76 nM	4.00	84%
V2-V4	I408T	R5	5.76 nM	4.00	84%
V3-V4	L317W	R5	4.29 nM	3.00	85%
V3-V4	I408T	R5	4.29 nM	3.00	85%
V2-V3-V4	V169M	R5	4.88 nM	3.31	83%
	L317W
	I408T

IC₅₀, 50% effective concentration or concentration needed to inhibit 50% of HIV. IC₅₀ FC was calculated as the ratio IC₅₀ for resistant virus/IC₅₀ for wild type virus. MPI (maximum percent inhibition) was calculated as described in Methods.