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qRT-PCR validation of five differentially expressed miRNAs identified using Illumina small RNA deep sequencing. A. Fold-change of five miRNAs that were differentially expressed between 42-d and 162-d ovaries based on deep sequencing data. B. The relative expression abundance of the five miRNAs between 42-d and 162- d ovaries by real-time quantitative RT-PCR. * P < 0.05,** P < 0.01.
