Figure 5.

Caspase activity inhibition did not rescue the effects of genistein’s effect on recovering HeLa cells. (a). HeLa cells were stressed or unstressed with 5.5% ethanol for 8 h (E8 hrs), in the absence (−) or the presence (+) of 25, 50, and 100 μM of general caspase inhibitor Z-VAD-fmk, and the PARP cleavage was examined by Western blot. (b). MTT assay about genistein’s effects on stressed HeLa cell, in the absence (−) or the presence (+) of general caspase inhibitor Z-VAD-fmk (100 μM) for 24 h. E 8 hrs stands for 8 h ethanol stress; Washing stands for the removal of ethanol-containing medium (Data expressed as Mean ± SEM, n = 3; ns indicates: no significance; ** indicates: p < 0.01).