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(A) PAGE; preparations were separated by discontinuous Tris-glycine 13% SDS-PAGE. For Mw determination Page Ruler Prestained Protein ladder (Fermentas SM0671) was used for Mw determination (lane M). Purified virus aliquots of 1 µg (1) and 10 µg (2) per lane were used. (B) Electron microscopy of sap from PVA-infected N. benthamiana; magnification ×20,000.
