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. 2013 Jun 28;8:2351–2360. doi: 10.2147/IJN.S42617

Figure 4.

Figure 4

Effect of DOPE in liposomes on the degree of fluid liposomes-bacterium fusion at 37°C for 30 minutes.

Notes: (A) Without the addition of calcium. (B) With the addition of calcium. Data are shown as means ± standard deviation (n = 3). *P < 0.05. Fluid liposomes with lipid composition of DPPC/DOPE/DMPG/NBD-PE/Rh-PE (the percentage of DMPGat 10% molar ratio, NBD-PE and Rh-PE each at 0.5% were kept constant) were prepared using the hydration-extrusion method. The vesicles were extruded through specific sized filters ten times. Pseudomonas aeruginosa 25619 was used in this study.

Abbreviations: DMPG, 1,2-dimyristoyl-sn-glycero-3-phosphoglycerol sodium salt; DOPE, 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine; DPPC, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine; NBD-PE, 1,2-dioleoyl-sn-Glycero-3-pHospHoetHanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl); RH-PE, 1,2-dioleoyl-sn-Glycero-3-pHospHoetHanolamine-N-(lissamine RHodamine B sulfonyl).