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. 2013 Apr 4;5(6):1872–1876. doi: 10.3892/ol.2013.1291

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Copyright © 2013, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Cells of group F were cultured in flavopiridol for 48 h. Group R received 6 Gy radiation. Group FR also received 6 Gy radiation after the cells had been cultured in flavopiridol for 48 h. After radiation, the cells were cultured another day. Then the cells were washed with PBS, collected with trypsinization, stained with Annexin V/propidium iodide and (A) analyzed using flow cytometry. This experiment was conducted 3 times. (B) was calculated using SPSS 13.0. The group control showed 15.53±5.40% apoptosisi, F showed 15.50±7.95%, R showed 22.76±9.71% and FR showed 37.92±16.15%. The apoptosis of FR was evidently greater than the other 3 groups. The difference was statistically significant (P<0.05). C, control; F, flavopiridol; R, radiation only; FR, flavopiridol with radiation.