Table 1. Oligonucleotides used in this studya.
Name | Sequence (5′-3′) |
ospC U866F | AGCTTAATTTTTTCCACAATGG |
ospC D697R+AatII+AgeI | ACCGGT AATGACGTCTGACTTATATTGACTTTATTTTTCCAG |
ospC D673F+AatII | GACGTC GGAAAAATAAAGTCAATATAAGTCAAG |
ospC D1572R+AgeI | ACCGGT AATGGAAAAATTCCTAATGTCG |
ospC U291F | ATTAGTTGGCTATATTGGG |
kanR 488R | TCACTCGCATCAACCAAACC |
ospCp mutHup(297)F | TAAGACAATATTGAAAAAATTCTTCAAT |
ospCp mutHup(297)R | ATTGAAGAATTTTTTCAATATTGTCTTA |
ospCp mutDH(297)R2 | TTTCAATTTTTTATTTTTTCAAATATTTGAAAAAATTCTTCAATATTT |
flaB 423F | TTCTCAAAATGTAAGAACAGCTGAAGA |
flaB 542R | TGGTTTGTCCAACATGAACTC |
flaB probe | 6-FAM-TCACTTTCAGGGTCTCAAGCGTCTTGGAC-TAMRA |
ospC F | CATGGGCAACTTGGAATTGA |
ospC R | TTGCCAAGTTTTCTACTGCTTTAAATAG |
ospC probe | 6-FAM-TAAAGATAAGGGCGCTGCAGAGC-TAMRA |
Synthetic restriction sites are underlined.