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. 2013 Jul 3;8(7):e68799. doi: 10.1371/journal.pone.0068799

Table 1. Oligonucleotides used in this studya.

Name Sequence (5′-3′)
ospC U866F AGCTTAATTTTTTCCACAATGG
ospC D697R+AatII+AgeI ACCGGT AATGACGTCTGACTTATATTGACTTTATTTTTCCAG
ospC D673F+AatII GACGTC GGAAAAATAAAGTCAATATAAGTCAAG
ospC D1572R+AgeI ACCGGT AATGGAAAAATTCCTAATGTCG
ospC U291F ATTAGTTGGCTATATTGGG
kanR 488R TCACTCGCATCAACCAAACC
ospCp mutHup(297)F TAAGACAATATTGAAAAAATTCTTCAAT
ospCp mutHup(297)R ATTGAAGAATTTTTTCAATATTGTCTTA
ospCp mutDH(297)R2 TTTCAATTTTTTATTTTTTCAAATATTTGAAAAAATTCTTCAATATTT
flaB 423F TTCTCAAAATGTAAGAACAGCTGAAGA
flaB 542R TGGTTTGTCCAACATGAACTC
flaB probe 6-FAM-TCACTTTCAGGGTCTCAAGCGTCTTGGAC-TAMRA
ospC F CATGGGCAACTTGGAATTGA
ospC R TTGCCAAGTTTTCTACTGCTTTAAATAG
ospC probe 6-FAM-TAAAGATAAGGGCGCTGCAGAGC-TAMRA
a

Synthetic restriction sites are underlined.