Figure 5. Metabolite time series obtained during glucose metabolism in non-growing cells of L. lactis.

Cells grown at 6.5 (open diamonds) or 5.1 (filled diamonds). The metabolite pools were monitored online by in vivo ¹³C-NMR at 30 ^°C, under anaerobic conditions and pH controlled at 5.1 in both cases. (A) Kinetics of [1-¹³C] glucose (40 mM) consumption, (B) extracellular lactic acid formation, (C) pools of fructose 1,6-bisphosphate, and (D) profiles of intracellular lactic acid. The glucose consumption rate was 0.22 and 0.34 µmol min^-1 mg protein^-1 for non-adapted and adapted cells, respectively. Each experiment was performed twice with good reproducibility.