Table 1. Maximal levels of glycolytic intermediates and glucose consumption rate during glucose metabolism by non-growing cells of L. lactis MG1363 at different pH conditions.
| Growth pH | External pH | FBPmax (mM) | 3-PGA (mM) | PEP (mM) | T6P (mM) | GCRmax (µmol min-1 mg protein -1) | |
|---|---|---|---|---|---|---|---|
| 6.5 | 6.5 | 46.6±3.8 | 9.1±1.5 | 6.5±0.4# | ND | 0.38±0.03 | |
| 5.5 | 33.3±3.0 | 6.0±1.8 | ND | 1.6±0.5 | 0.30±0.04 | ||
| 5.1 | 27.6±1.3 | 5.1±1.2# | ND | 1.4±0.1 | 0.22±0.02 | ||
| 4.8 | 22.4±1.8 | ND | ND | 1.0±0.1 | 0.16±0.01 | ||
| 5.1 | 5.1 | 37.5±6.7 | 5.2±1.0# | ND | ND | 0.34±0.05 | |
The values are averages of at least two independent experiments.
# Values determined by 13C-NMR in crude cell extracts obtained from the cell suspensions used in the in vivo NMR experiments. ND, not detected in vivo and in cell extracts.