Table 1. Primers used for real-timer PCR experiments.
Primers | Sequences |
---|---|
IFN-γ | Forward: 5’- GGCTGTCCCTGAAAGAAAGC -3’ Reverse: 5’- GAGCGAGTTATTTGTCATTCGG -3’ |
TNF-α | Forward: 5’- AAGGGAGAGTGGTCAGGTTGCC -3’ Reverse: 5’- CCTCAGGGAAGAGTCTGGAAAGG -3’ |
IFN-α | Forward: 5’- GACTTTGGATTTCCCCTGGAG -3’ Reverse: 5’- AAGCCTTTGATGTGAAGAGGTTC -3’ |
IFN-β | Forward: 5’- TACGTCTCCTGGATGAACTC-3’ Reverse: 5’- TCTTCAAGTGGAGAGCAGTT -3’ |
IL-6 | Forward: 5'- GTCGGAGGCTTAATTACACA -3' Reverse: 5'- TGCATCATCGTTGTTCATAC -3' |
IL-10 | Forward: 5'- CGCTGTCATCGATTTCTC -3' Reverse: 5'- ATTCATGGCCTTGTAGACAC -3' |
IL-18 | Forward: 5'- AAATGGAGACCTGGAATCAGAC -3' Reverse: 5'- TTTGTCAACGAAGAGAACTTGG -3' |
GAPDH | Forward: 5’- TACACTGAGGACCAGGTTGT -3’ Reverse: 5’- CAAAGTTGTCATTGAGAGCA -3’ |