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. 2013 Jul 3;8(7):e68640. doi: 10.1371/journal.pone.0068640

Figure 2. TLR-2 and TLR-4 but not TREM-1, regulate activation of macrophages in vitro by kidney DAMPs.

Figure 2

(A–B) Q-PCR showing Il-1β and Trem1 expression in BMDMφ stimulated for 8, 16 or 24h with soluble factors prepared from normal kidney (control) or disease kidney (kidney DAMPs). (C–D) Graphs showing Il-1β and Trem1 expression by Q–PCR, 16h after BMDMφ were stimulated with kidney DAMPs and (C) activating anti-TREM-1 antibodies, or (D) TREM1-Fc, which blocks TREM-1 receptor by competing for ligands. (E–F) Q-PCR showing (E) Il-1β and (F) Trem1 expression in BMDMφ isolated from WT, Tlr2−/−, Tlr4−/−, Tlr2–4−/−, and Myd88−/− mice stimulated with kidney DAMPs for 16h. Q-PCR results were normalized to their respective control group. (*P<0.05, n = 5–7/group, 3 independent experiments; ns, p is not significant).