Figure 2. CX₃CR1 deficiency does not influence the replenishment of retinal microglia or vitreal hyalocytes in BALB/c mice.

At 4 weeks post BM transfer, donor GFP⁺ cells were present in small numbers surrounding the optic disc (OD) at the level of the NFL (A), inner plexiform layer (not shown) and OPL (C) of both Cx ₃ cr1 ^+/gfp → WT and Cx ₃ cr1 ^gfp/gfp → WT chimeric mice. At week 8, the proportion of GFP⁺ cells appeared to be similar in Cx ₃ cr1 ^+/gfp → WT (B, D; left) and Cx ₃ cr1 ^gfp/gfp → WT chimeric mice (B, D; right). The cell density of donor GFP⁺ vitreal hyalocytes in Cx ₃ cr1 ^+/gfp → WT and Cx ₃ cr1 ^gfp/gfp → WT chimeric mice was similar at 8 weeks post BM transfer (E; P = 0.12). Examples of hyalocytes indicated by white arrows (F). n= 4 mice per group.

NFL, nerve fiber layer; OPL, outer plexiform layer.