Figure 1. Western blot analysis of PrP^C from normal human tissues.

Ten percent homogenates from normal human brain (Br), spleen (Sp), tonsil (Ts), and lymph node (LN) were prepared and ultracentrifuged through a sucrose cushion as described in Materials and Methods. Pellets were resuspended and either untreated or PK-treated prior to western blot analysis with Mab 3F4.