Figure 1.

Overexpression of ECRG4 in M2 cells. The reconstructive plasmid pFLAG-CMV-2-ECRG4 was stably transfected into the M2 cells. Real-time PCR and western blot analysis were applied to detect the transfection efficiency. Briefly, the cells without interference or those cells transfected with pFLAG-CMV-2 and pFLAG-CMV-2-ECRG4 were referred to as the control, vector and ECRG4, respectively. (A) Increased protein expression of ECRG4 detected by western blot analysis. (B) Increased mRNA expression of ECRG4 as revealed by RT-PCR. ^*P<0.05 vs. control group.