Table II.
Correlation between inflammatory infiltrate intensity and expression of cluster of differentiation 8 (CD8), forkhead box P3 (FOXP3), transforming growth factor-β (TGF-β), tumor necrosis factor-α (TNF-α) and nuclear factor-κ-light chain enhancer of activated B-cells (NF-κB).
| Inflammatory infiltrate intensity | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
| ||||||||||||
| Dysplasia (n=20) | Carcinoma (n=40) | |||||||||||
|
|
|
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| Expression (%) | Mild n (%) | Mod. n (%) | Int. n (%) | Total | r | P-value | Mild n (%) | Mod. n (%) | Int. n (%) | Total | r | P-value |
| CD8 | ||||||||||||
| <5 | 2 (40) | 1 (10) | 1 (20) | 4 (20) | 11 (100) | 6 (60) | 10 (50) | 27 (67.5) | ||||
| 5–50 | 2 (40) | 2 (20) | 0 (0) | 4 (20) | 0.391 | NS | 0 (0) | 1 (10) | 2 (10) | 3 (7.5) | 0.365 | <0.05a |
| >50 | 1 (20) | 7 (70) | 4 (80) | 12 (60) | 0 (0) | 2 (20) | 8 (40) | 10 (25) | ||||
| FOXP3 | ||||||||||||
| <5 | 3 (60) | 6 (60) | 2 (40) | 11 (55) | 7 (63.6) | 6 (60) | 11 (57.9) | 24 (60) | ||||
| 5–50 | 1 (20) | 2 (20) | 2 (40) | 5 (25) | 0.109 | NS | 2 (18.2) | 3 (30) | 6 (31.6) | 11 (27.5) | 0.018 | NS |
| >50 | 1 (20) | 2 (20) | 1 (20) | 4 (20) | 2 (18.2) | 1 (10) | 2 (10.5) | 5 (12.5) | ||||
| TGF-β (epithelial cells) | ||||||||||||
| <5 | 1 (20) | 3 (30) | 0 (0) | 4 (20) | 4 (36.4) | 3 (30) | 9 (47.4) | 16 (40) | ||||
| 5–50 | 1 (20) | 4 (40) | 3 (60) | 8 (40) | 0.026 | NS | 3 (27.2) | 5 (50) | 6 (31.6) | 14 (35) | −0.142 | NS |
| >50 | 3 (60) | 3 (40) | 2 (40) | 8 (40) | 4 (36.4) | 2 (20) | 4 (21) | 10 (25) | ||||
| TGF-β (stromal cells) | ||||||||||||
| <5 | 0 (0) | 4 (40) | 0 (0) | 4 (20) | 3 (27.2) | 2 (20) | 2 (10.5) | 7 (17.5) | ||||
| 5–50 | 2 (40) | 1 (10) | 2 (40) | 5 (25) | 0.000 | NS | 4 (36.4) | 3 (30) | 8 (42.1) | 15 (37.5) | 0.189 | NS |
| >50 | 3 (60) | 5 (50) | 3 (60) | 11 (55) | 4 (36.4) | 5 (50) | 9 (47.4) | 18 (45) | ||||
| TNF-α (epithelial cells) | ||||||||||||
| <5 | 3 (60) | 2 (20) | 1 (20) | 6 (30) | 3 (27.3) | 1 (10) | 10 (52.6) | 14 (35) | ||||
| 5–50 | 2 (40) | 4 (40) | 1 (20) | 7 (35) | 0.442 | <0.05a | 6 (54.5) | 6 (60) | 6 (31.6) | 18 (45) | −0.337 | <0.05a |
| >50 | 0 (0) | 4 (40) | 3 (60) | 7 (35) | 2 (18.2) | 3 (30) | 3 (15.8) | 8 (20) | ||||
| TNF-α (stromal cells) | ||||||||||||
| <5 | 2 (40) | 1 (10) | 0 (0) | 3 (15) | 9 (81.8) | 3 (30) | 9 (47.4) | 21 (52.5) | ||||
| 5–50 | 3 (60) | 6 (60) | 1 (20) | 10 (50) | 0.632 | <0.05a | 1 (9.1) | 5 (50) | 6 (31.6) | 12 (30) | 0.383 | <0.05a |
| >50 | 0 (0) | 3 (30) | 4 (80) | 7 (35) | 1 (9.1) | 2 (20) | 4 (21) | 7 (17.5) | ||||
| NF-κB | ||||||||||||
| <5 | 4 (80) | 2 (20) | 0 (0) | 6 (30) | 6 (54.5) | 6 (60) | 8 (42.1) | 20 (50) | ||||
| 5–50 | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0.617 | <0.05a | 3 (27.3) | 2 (20) | 10 (52.6) | 15 (37.5) | 0.046 | NS |
| >50 | 1 (20) | 8 (80) | 5 (100) | 14 (70) | 2 (18.2) | 2 (20) | 1 (5.3) | 5 (12.5) | ||||
Spearman rank correlation coefficient.
a
P<0.05, statistically significant; NS, not significant. Mod., moderate; Int., intense.