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. 2013 Jul 3;8(7):e67765. doi: 10.1371/journal.pone.0067765

Figure 5. Expression of CCp1-3 during acute B. bovis infection.

Figure 5

In panel A, relative fold expression of CCp1-3 transcripts in bovine blood during acute B. bovis infection is shown in the right y-axis. Absolute quantification of B. bovis msa-1 DNA in bovine blood is shown in the left y-axis. CCp gene expression was normalized to the total amount of RNA used to generate the cDNA and the transcription level was calculated as a relative expression using the formula: Relative expression (sample) = 2 [Cq (control) – Cq (sample)], where the control is the highest Cq value for a given gene of interest. To confirm the presence of amplifiable cDNA in all tested samples a standard PCR for bovine actin was performed. In panel B, absolute expression of B. bovis msa-1 RNA in bovine blood is shown in the right y-axis and the percentage of engorged ticks infected with B. bovis is shown in the left y-axis.