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. 2013 Jul 3;8(7):e67765. doi: 10.1371/journal.pone.0067765

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© 2013 Bastos et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Relative fold expression of CCp1-3 transcripts in horse peripheral blood during acute T. equi infection is shown in the right y-axis. Absolute quantification of T. equi emai-1 DNA (black line) and cDNA (grey line) is shown in the left y-axis. CCp gene expression was normalized to the total amount of RNA used to generate the cDNA and the transcription level was calculated as a relative expression using the formula: Relative expression _(sample) = 2 ^{[Cq (control) – Cq (sample)]}, where the control is the highest Cq value for a given gene of interest. To confirm the presence of amplifiable cDNA in all tested samples a standard PCR for horse actin was performed.