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Real-time PCR measurements of erythropoietin (Epo) and Epo receptor (EpoR) expression in renal cell carcinoma (RCC) cell lines. A total of five cell lines were used; 769P, 786O, Ku19/20, Caki-1 and SKRC44. Total RNA was extracted and analyzed for Epo and EpoR expression by real-time PCR. Caki-1 and SKRC44 cells highly express both Epo and EpoR. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the housekeeping control gene.
