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Erythropoietin (EPO)-induced cell growth in renal cell carcinoma (RCC) cell lines. Proliferation of 769P, 786O, Ku19/20, Caki-1 and SKRC44 cells in the presence of fetal bovine serum (FBS; 10%) or exogenous erythropoietin (0, 0.033 and 0.1 ng/ml) is shown in the bar graph. Caki-1 and SKRC44 cells highly express Epo. The proliferation rate of each cell line was measured using the water-soluble tetrazolium salt-1 (WST-1) assay as described in the Materials and methods.
